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APC anti-mouse CD45.1 Antibody APC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20_APC_090507
SJL mouse splenocytes stained with A20 APC
  • A20_APC_090507
    SJL mouse splenocytes stained with A20 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
110714 100 µg NOK1048
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110713 25 µg NOK363
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  4. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  5. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  6. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  7. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  8. Durgin JS, et al. 2022. Mol Ther. 30:1201. PubMed
  9. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  10. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  13. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  14. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  15. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  16. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  17. Nixon BG, et al. 2023. STAR Protoc. 4:102185. PubMed
  18. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  19. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  20. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  21. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  22. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  23. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  24. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  25. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  26. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  27. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  28. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  29. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  30. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  31. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  32. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  33. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  34. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  35. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  36. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  37. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  38. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  39. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  40. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  41. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  42. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  43. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  44. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  45. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  46. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  47. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  48. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  49. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  50. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  51. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  52. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  53. Li J, et al. 2020. Elife. 9:00. PubMed
  54. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  55. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  56. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  57. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  58. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  59. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  60. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  61. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  62. Port JR, et al. 2020. J Virol. 94:. PubMed
  63. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  64. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  65. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  66. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  67. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  68. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  69. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  70. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  71. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  72. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  73. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  74. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  75. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  76. Ocaña-Morgner C, et al. 2013. J Immunol. 190:5545. PubMed
  77. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  78. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  79. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  80. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  81. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  82. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  83. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  84. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  85. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  86. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  87. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  88. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  89. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  90. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  91. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  92. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  93. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  94. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  95. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  96. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  97. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  98. Durgin JS, et al. 2022. Mol Ther. 30:1201. PubMed
  99. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  100. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  101. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  102. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  103. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  104. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  105. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  106. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  107. Nixon BG, et al. 2023. STAR Protoc. 4:102185. PubMed
  108. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  109. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  110. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  111. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  112. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  113. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  114. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  115. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  116. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  117. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  118. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  119. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  120. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  121. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  122. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  123. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  124. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  125. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  126. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  127. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  128. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  129. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  130. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  131. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  132. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  133. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  134. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  135. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  136. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  137. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  138. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  139. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  140. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  141. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  142. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  143. Li J, et al. 2020. Elife. 9:00. PubMed
  144. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  145. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  146. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  147. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  148. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  149. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  150. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  151. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  152. Port JR, et al. 2020. J Virol. 94:. PubMed
  153. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  154. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  155. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  156. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  157. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  158. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  159. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  160. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  161. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  162. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  163. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  164. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  165. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  166. Ocaña-Morgner C, et al. 2013. J Immunol. 190:5545. PubMed
  167. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  168. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  169. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  170. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  171. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  172. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  173. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  174. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  175. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  176. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  177. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  178. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  179. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  180. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
RRID
AB_313503 (BioLegend Cat. No. 110714)
AB_313502 (BioLegend Cat. No. 110713)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
PE anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC anti-mouse CD45.1 A20 FC
APC/Cyanine7 anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20_APC_090507
SJL mouse splenocytes stained with A20 APC
  • A20_APC_090507
    SJL mouse splenocytes stained with A20 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
110714 100 µg NOK1048
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110713 25 µg NOK363
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
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Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  3. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  4. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  5. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  6. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  7. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  8. Durgin JS, et al. 2022. Mol Ther. 30:1201. PubMed
  9. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  10. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  13. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  14. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  15. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  16. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  17. Nixon BG, et al. 2023. STAR Protoc. 4:102185. PubMed
  18. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  19. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  20. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  21. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  22. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  23. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
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  25. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  26. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  27. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  28. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  29. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
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  32. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  33. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
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  35. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  36. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
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  40. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  41. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  42. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  43. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
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  45. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  46. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
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  48. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  49. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  50. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  51. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  52. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  53. Li J, et al. 2020. Elife. 9:00. PubMed
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  55. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
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  57. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  58. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  59. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  60. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  61. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  62. Port JR, et al. 2020. J Virol. 94:. PubMed
  63. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  64. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  65. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  66. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  67. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  68. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  69. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  70. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  71. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
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  79. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  80. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  81. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  82. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  83. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  84. Sun Y, et al. 2022. iScience. 25:104846. PubMed
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  86. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  87. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  88. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  89. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  90. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  91. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  92. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  93. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  94. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  95. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  96. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  97. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  98. Durgin JS, et al. 2022. Mol Ther. 30:1201. PubMed
  99. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  100. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  101. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  102. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  103. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  104. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
  105. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  106. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  107. Nixon BG, et al. 2023. STAR Protoc. 4:102185. PubMed
  108. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  109. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  110. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  111. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  112. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  113. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  114. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  115. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  116. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  117. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  118. Bangs DJ, et al. 2022. Cell Rep. 38:110266. PubMed
  119. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  120. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  121. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  122. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  123. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  124. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  125. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  126. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  127. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  128. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  129. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  130. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  131. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  132. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  133. García-Bonilla M, et al. 2020. Stem Cell Res Ther. 11:121. PubMed
  134. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  135. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  136. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  137. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  138. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  139. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  140. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  141. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  142. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  143. Li J, et al. 2020. Elife. 9:00. PubMed
  144. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  145. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  146. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  147. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  148. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  149. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  150. Wang L, et al. 2014. Proc Natl Acad Sci U S A. 111:3146. PubMed
  151. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  152. Port JR, et al. 2020. J Virol. 94:. PubMed
  153. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  154. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  155. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  156. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
  157. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  158. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  159. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  160. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  161. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  162. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  163. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  164. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  165. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  166. Ocaña-Morgner C, et al. 2013. J Immunol. 190:5545. PubMed
  167. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  168. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  169. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  170. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  171. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  172. Akane K, et al. 2016. Proc Natl Acad Sci U S A. 113: 2460 - 2465. PubMed
  173. Hu Z, et al. 2013. J Immunol. 191:312. PubMed
  174. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  175. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  176. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  177. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  178. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  179. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  180. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
RRID
AB_313503 (BioLegend Cat. No. 110714)
AB_313502 (BioLegend Cat. No. 110713)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
PE anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC anti-mouse CD45.1 A20 FC
APC/Cyanine7 anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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