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PE anti-mouse CD45.1 Antibody PE anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20
SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
  • A20
    SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
110708 200 µg NOK1766
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110707 50 µg NOK660
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  2. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  3. Albayrak E, et al. 2022. J Cell Biochem. 123:2009. PubMed
  4. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  5. Hägglöf T, et al. 2023. Cell. 186:147. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  8. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  9. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  10. Li X, et al. 2023. J Clin Invest. 133:. PubMed
  11. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  12. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  13. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  14. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  15. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  16. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  17. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  18. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  19. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  20. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  21. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  22. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  23. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  24. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  25. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  26. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  27. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  28. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  29. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  30. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  31. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  32. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  33. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  34. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  35. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  36. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  37. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  38. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  39. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  40. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  41. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  42. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  43. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  44. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  45. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  46. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  47. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  48. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  49. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  50. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  51. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  52. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  53. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  54. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  55. Bei T, et al. 2021. Molecules. 26:. PubMed
  56. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  57. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  58. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  59. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  60. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  61. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  62. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  63. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  64. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  65. Ma K, et al. 2022. iScience. 25:104347. PubMed
  66. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  67. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  68. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  69. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  70. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  71. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  72. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  73. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  74. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  75. Danzer H, et al. 2020. Elife. 9:00. PubMed
  76. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  77. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  78. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  79. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  80. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  81. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  82. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  83. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  84. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  85. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  86. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  87. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  88. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  89. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  90. Albayrak E, et al. 2022. J Cell Biochem. 123:2009. PubMed
  91. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  92. Hägglöf T, et al. 2023. Cell. 186:147. PubMed
  93. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  94. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  95. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  96. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  97. Li X, et al. 2023. J Clin Invest. 133:. PubMed
  98. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  99. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  100. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  101. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  102. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  103. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  104. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  105. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  106. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  107. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  108. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  109. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  110. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  111. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  112. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  113. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  114. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  115. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  116. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  117. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  118. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  119. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  120. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  121. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  122. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  123. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  124. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  125. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  126. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  127. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  128. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  129. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  130. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  131. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  132. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  133. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  134. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  135. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  136. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  137. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  138. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  139. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  140. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  141. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  142. Bei T, et al. 2021. Molecules. 26:. PubMed
  143. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  144. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  145. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  146. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  147. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  148. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  149. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  150. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  151. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  152. Ma K, et al. 2022. iScience. 25:104347. PubMed
  153. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  154. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  155. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  156. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  157. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  158. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  159. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  160. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  161. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  162. Danzer H, et al. 2020. Elife. 9:00. PubMed
  163. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  164. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  165. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  166. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  167. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  168. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  169. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  170. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  171. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  172. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  173. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  174. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_313497 (BioLegend Cat. No. 110708)
AB_313496 (BioLegend Cat. No. 110707)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC anti-mouse CD45.1 A20 FC
APC/Cyanine7 anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20
SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
  • A20
    SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
110708 200 µg NOK1766
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110707 50 µg NOK660
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
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Product Citations
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  11. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
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  19. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
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  89. Miranda K, et al. 2022. iScience. 25:104994. PubMed
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  91. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  92. Hägglöf T, et al. 2023. Cell. 186:147. PubMed
  93. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  94. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  95. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  96. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  97. Li X, et al. 2023. J Clin Invest. 133:. PubMed
  98. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  99. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  100. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  101. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  102. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  103. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  104. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  105. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  106. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  107. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  108. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  109. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  110. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  111. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  112. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  113. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  114. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  115. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  116. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  117. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  118. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  119. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  120. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  121. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  122. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  123. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  124. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  125. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  126. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  127. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  128. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  129. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  130. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  131. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  132. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  133. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  134. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  135. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  136. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  137. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  138. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  139. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  140. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  141. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  142. Bei T, et al. 2021. Molecules. 26:. PubMed
  143. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  144. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  145. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  146. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  147. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  148. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  149. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  150. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  151. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  152. Ma K, et al. 2022. iScience. 25:104347. PubMed
  153. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  154. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  155. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  156. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  157. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  158. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  159. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  160. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  161. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  162. Danzer H, et al. 2020. Elife. 9:00. PubMed
  163. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  164. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  165. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  166. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  167. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  168. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  169. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  170. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  171. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  172. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  173. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  174. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_313497 (BioLegend Cat. No. 110708)
AB_313496 (BioLegend Cat. No. 110707)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC anti-mouse CD45.1 A20 FC
APC/Cyanine7 anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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