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FITC anti-mouse CD45.1 Antibody FITC anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_FITC_090507
SJL mouse splenocytes stained with A20 FITC
  • A20_FITC_090507
    SJL mouse splenocytes stained with A20 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
110705 50 µg NOK487
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110706 500 µg NOK908
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  4. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  5. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  6. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  7. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  8. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  9. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  10. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  11. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  12. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  13. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  14. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  15. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  16. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  17. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  18. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  19. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  22. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  23. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  24. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  25. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  26. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  27. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  28. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  29. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  30. Chen S, et al. 2022. Cancer Discov. :. PubMed
  31. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  32. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  33. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  34. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  35. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  36. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  37. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  38. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  39. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  40. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  41. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  42. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  43. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  44. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  45. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  46. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  47. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  48. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  49. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  50. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  51. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  52. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  53. He L, et al. 2019. iScience. 0.790972222. PubMed
  54. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  55. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  56. Ise W, et al. 2018. Immunity. 48:702. PubMed
  57. Lee S, et al. 2009. J Immunol. 182:6753. PubMed
  58. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  59. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  60. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  61. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  62. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  63. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  64. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  65. Wan X, et al. 2018. Nature. 560:107. PubMed
  66. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  67. Xu X, et al. 2018. Cell. 175:1336. PubMed
  68. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  69. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  70. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  71. Port JR, et al. 2020. J Virol. 94:. PubMed
  72. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  73. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  74. Jameson K 2009. J Exp Med. 206:2253. PubMed
  75. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  76. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  77. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  78. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  79. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  80. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  81. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  82. Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed
  83. Hata T, et al. 2013. J Immunol. 191:83. PubMed
  84. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  85. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  86. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  87. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  88. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  89. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  90. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  91. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  92. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  93. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  94. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  95. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  96. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  97. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  98. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  99. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  100. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  101. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  102. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  103. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  104. Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed
  105. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  106. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  107. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  108. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  109. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  110. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  111. Young K, et al. 2021. Cell Stem Cell. . PubMed
  112. Gay L, et al. 2013. Genes Dev. 27:98. PubMed
  113. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  114. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  115. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  116. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  117. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  118. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  119. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  120. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  121. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  122. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  123. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  124. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  125. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  126. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  127. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  128. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  129. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  130. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  131. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  132. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  133. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  134. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  135. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  136. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  137. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  138. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  139. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  140. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  141. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  142. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  143. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  144. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  145. Chen S, et al. 2022. Cancer Discov. :. PubMed
  146. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  147. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  148. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  149. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  150. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  151. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  152. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  153. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  154. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  155. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  156. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  157. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  158. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  159. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  160. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  161. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  162. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  163. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  164. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  165. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  166. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  167. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  168. He L, et al. 2019. iScience. 0.790972222. PubMed
  169. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  170. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  171. Ise W, et al. 2018. Immunity. 48:702. PubMed
  172. Lee S, et al. 2009. J Immunol. 182:6753. PubMed
  173. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  174. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  175. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  176. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  177. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  178. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  179. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  180. Wan X, et al. 2018. Nature. 560:107. PubMed
  181. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  182. Xu X, et al. 2018. Cell. 175:1336. PubMed
  183. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  184. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  185. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  186. Port JR, et al. 2020. J Virol. 94:. PubMed
  187. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  188. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  189. Jameson K 2009. J Exp Med. 206:2253. PubMed
  190. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  191. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  192. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  193. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  194. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  195. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  196. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  197. Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed
  198. Hata T, et al. 2013. J Immunol. 191:83. PubMed
  199. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  200. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  201. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  202. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  203. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  204. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  205. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  206. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  207. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  208. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  209. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  210. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  211. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  212. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  213. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  214. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  215. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  216. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  217. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  218. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  219. Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed
  220. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  221. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  222. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  223. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  224. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  225. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  226. Young K, et al. 2021. Cell Stem Cell. . PubMed
  227. Gay L, et al. 2013. Genes Dev. 27:98. PubMed
  228. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  229. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  230. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313494 (BioLegend Cat. No. 110705)
AB_313495 (BioLegend Cat. No. 110706)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC anti-mouse CD45.1 A20 FC
APC/Cyanine7 anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_FITC_090507
SJL mouse splenocytes stained with A20 FITC
  • A20_FITC_090507
    SJL mouse splenocytes stained with A20 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
110705 50 µg NOK487
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110706 500 µg NOK908
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  3. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  4. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  5. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  6. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  7. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  8. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  9. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  10. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  11. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  12. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  13. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  14. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  15. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  16. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  17. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  18. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  19. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  20. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  21. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  22. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  23. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  24. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  25. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  26. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  27. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  28. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  29. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  30. Chen S, et al. 2022. Cancer Discov. :. PubMed
  31. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  32. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  33. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  34. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  35. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  36. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  37. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  38. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  39. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  40. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  41. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  42. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  43. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  44. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  45. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  46. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  47. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  48. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  49. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  50. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  51. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  52. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  53. He L, et al. 2019. iScience. 0.790972222. PubMed
  54. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  55. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  56. Ise W, et al. 2018. Immunity. 48:702. PubMed
  57. Lee S, et al. 2009. J Immunol. 182:6753. PubMed
  58. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  59. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  60. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  61. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  62. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  63. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  64. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  65. Wan X, et al. 2018. Nature. 560:107. PubMed
  66. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  67. Xu X, et al. 2018. Cell. 175:1336. PubMed
  68. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  69. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  70. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  71. Port JR, et al. 2020. J Virol. 94:. PubMed
  72. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  73. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  74. Jameson K 2009. J Exp Med. 206:2253. PubMed
  75. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  76. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  77. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  78. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  79. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  80. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  81. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  82. Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed
  83. Hata T, et al. 2013. J Immunol. 191:83. PubMed
  84. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  85. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  86. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  87. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  88. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  89. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  90. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  91. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  92. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  93. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  94. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  95. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  96. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  97. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  98. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  99. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  100. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  101. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  102. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  103. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  104. Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed
  105. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  106. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  107. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  108. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  109. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  110. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  111. Young K, et al. 2021. Cell Stem Cell. . PubMed
  112. Gay L, et al. 2013. Genes Dev. 27:98. PubMed
  113. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  114. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  115. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  116. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  117. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  118. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  119. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  120. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  121. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  122. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  123. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  124. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  125. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  126. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  127. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  128. Ali LR, et al. 2023. J Exp Med. 220: . PubMed
  129. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  130. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  131. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  132. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  133. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  134. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  135. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  136. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  137. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  138. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  139. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  140. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  141. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  142. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  143. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  144. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  145. Chen S, et al. 2022. Cancer Discov. :. PubMed
  146. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  147. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  148. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  149. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  150. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  151. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  152. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  153. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  154. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  155. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  156. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  157. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  158. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  159. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  160. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  161. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  162. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  163. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  164. Sandrock I, et al. 2015. PLoS One. 10: 0145010. PubMed
  165. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  166. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  167. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  168. He L, et al. 2019. iScience. 0.790972222. PubMed
  169. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  170. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  171. Ise W, et al. 2018. Immunity. 48:702. PubMed
  172. Lee S, et al. 2009. J Immunol. 182:6753. PubMed
  173. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  174. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  175. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  176. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  177. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  178. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  179. Lee P, et al. 2014. J Immunol. 192:178. PubMed
  180. Wan X, et al. 2018. Nature. 560:107. PubMed
  181. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  182. Xu X, et al. 2018. Cell. 175:1336. PubMed
  183. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  184. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  185. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  186. Port JR, et al. 2020. J Virol. 94:. PubMed
  187. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  188. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  189. Jameson K 2009. J Exp Med. 206:2253. PubMed
  190. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  191. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  192. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  193. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  194. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  195. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  196. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  197. Fallet B, et al. 2016. Immunology. 1: eaah6817. PubMed
  198. Hata T, et al. 2013. J Immunol. 191:83. PubMed
  199. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  200. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  201. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  202. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  203. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  204. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  205. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  206. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  207. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  208. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  209. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  210. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  211. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  212. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  213. Tanaka S, et al. 2021. Am J Transplant. 21:353. PubMed
  214. Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
  215. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  216. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  217. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  218. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  219. Huang L, et al. 2015. PLoS Pathog. 11: 1005347. PubMed
  220. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  221. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  222. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  223. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  224. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  225. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  226. Young K, et al. 2021. Cell Stem Cell. . PubMed
  227. Gay L, et al. 2013. Genes Dev. 27:98. PubMed
  228. Cabal-Hierro L, et al. 2020. Nat Commun. 11:1406. PubMed
  229. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  230. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313494 (BioLegend Cat. No. 110705)
AB_313495 (BioLegend Cat. No. 110706)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC anti-mouse CD45.1 A20 FC
APC/Cyanine7 anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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