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APC anti-mouse CD45.2 Antibody APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg SEK278
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109814 100 µg SEK975
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  2. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  3. He D, et al. 2022. Immunity. 55:159. PubMed
  4. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  5. Campisi L, et al. 2022. Nature. 606:945. PubMed
  6. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  7. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  8. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  9. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  10. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  13. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  14. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  15. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  16. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  17. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  18. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  19. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  20. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  21. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  22. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  23. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  24. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  25. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  26. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  27. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  28. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  29. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  30. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  31. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  32. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  33. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  34. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  35. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  36. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  37. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  38. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  39. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  40. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  41. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  42. Lu SX, et al. 2021. Cell. . PubMed
  43. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  44. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  45. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  46. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  47. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  48. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  49. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  50. Marangoni F, et al. 2021. Cell. . PubMed
  51. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  52. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  53. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  56. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  57. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  58. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  59. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  60. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  61. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  62. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  63. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  64. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  65. Markey K, et al. 2012. Blood. 119:5918. PubMed
  66. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  67. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  68. Xu X, et al. 2018. Cell. 175:1336. PubMed
  69. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  70. Bei T, et al. 2021. Molecules. 26:. PubMed
  71. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  72. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  73. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  74. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  75. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  76. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  77. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  78. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  79. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  80. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  81. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  82. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  83. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  84. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  85. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  86. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  87. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  88. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  89. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  90. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  91. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  92. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  93. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  94. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  95. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  96. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  97. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  98. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  99. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  100. Koide S, et al. 2022. iScience. 25:103603. PubMed
  101. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  102. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  103. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  104. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  105. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  106. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  107. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  108. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  109. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  110. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  111. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  112. Wang X, et al. 2022. Elife. 11:. PubMed
  113. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  114. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  115. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  116. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  117. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  118. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  119. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  120. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  121. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  122. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  123. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  124. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  125. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  126. He D, et al. 2022. Immunity. 55:159. PubMed
  127. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  128. Campisi L, et al. 2022. Nature. 606:945. PubMed
  129. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  130. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  131. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  132. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  133. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  134. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  135. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  136. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  137. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  138. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  139. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  140. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  141. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  142. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  143. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  144. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  145. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  146. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  147. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  148. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  149. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  150. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  151. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  152. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  153. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  154. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  155. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  156. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  157. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  158. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  159. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  160. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  161. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  162. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  163. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  164. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  165. Lu SX, et al. 2021. Cell. . PubMed
  166. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  167. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  168. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  169. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  170. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  171. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  172. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  173. Marangoni F, et al. 2021. Cell. . PubMed
  174. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  175. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  176. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  179. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  180. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  181. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  182. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  183. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  184. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  185. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  186. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  187. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  188. Markey K, et al. 2012. Blood. 119:5918. PubMed
  189. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  190. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  191. Xu X, et al. 2018. Cell. 175:1336. PubMed
  192. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  193. Bei T, et al. 2021. Molecules. 26:. PubMed
  194. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  195. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  196. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  197. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  198. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  199. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  200. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  201. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  202. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  203. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  204. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  205. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  206. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  207. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  208. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  209. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  210. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  211. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  212. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  213. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  214. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  215. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  216. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  217. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  218. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  219. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  220. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  221. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  222. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  223. Koide S, et al. 2022. iScience. 25:103603. PubMed
  224. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  225. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  226. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  227. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  228. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  229. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  230. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  231. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  232. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  233. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  234. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  235. Wang X, et al. 2022. Elife. 11:. PubMed
  236. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  237. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  238. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  239. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  240. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  241. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  242. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  243. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  244. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  245. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  246. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg SEK278
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109814 100 µg SEK975
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  2. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  3. He D, et al. 2022. Immunity. 55:159. PubMed
  4. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  5. Campisi L, et al. 2022. Nature. 606:945. PubMed
  6. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  7. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  8. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  9. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  10. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  13. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  14. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  15. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  16. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  17. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  18. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  19. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  20. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  21. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  22. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  23. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  24. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  25. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  26. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  27. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  28. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  29. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  30. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  31. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  32. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  33. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  34. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  35. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  36. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  37. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  38. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  39. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  40. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  41. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  42. Lu SX, et al. 2021. Cell. . PubMed
  43. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  44. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  45. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  46. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  47. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  48. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  49. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  50. Marangoni F, et al. 2021. Cell. . PubMed
  51. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  52. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  53. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  56. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  57. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  58. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  59. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  60. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  61. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  62. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  63. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  64. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  65. Markey K, et al. 2012. Blood. 119:5918. PubMed
  66. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  67. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  68. Xu X, et al. 2018. Cell. 175:1336. PubMed
  69. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  70. Bei T, et al. 2021. Molecules. 26:. PubMed
  71. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  72. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  73. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  74. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  75. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  76. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  77. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  78. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  79. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  80. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  81. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  82. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  83. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  84. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  85. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  86. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  87. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  88. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  89. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  90. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  91. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  92. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  93. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  94. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  95. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  96. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  97. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  98. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  99. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  100. Koide S, et al. 2022. iScience. 25:103603. PubMed
  101. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  102. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  103. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  104. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  105. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  106. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  107. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  108. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  109. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  110. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  111. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  112. Wang X, et al. 2022. Elife. 11:. PubMed
  113. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  114. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  115. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  116. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  117. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  118. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  119. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  120. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  121. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  122. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  123. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  124. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  125. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  126. He D, et al. 2022. Immunity. 55:159. PubMed
  127. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  128. Campisi L, et al. 2022. Nature. 606:945. PubMed
  129. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  130. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  131. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  132. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  133. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  134. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  135. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  136. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  137. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  138. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  139. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  140. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  141. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  142. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  143. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  144. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  145. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  146. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  147. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  148. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  149. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  150. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  151. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  152. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  153. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  154. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  155. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  156. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  157. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  158. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  159. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  160. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  161. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  162. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  163. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  164. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  165. Lu SX, et al. 2021. Cell. . PubMed
  166. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  167. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  168. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  169. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  170. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  171. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  172. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  173. Marangoni F, et al. 2021. Cell. . PubMed
  174. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  175. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  176. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  179. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  180. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  181. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  182. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  183. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  184. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  185. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  186. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  187. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  188. Markey K, et al. 2012. Blood. 119:5918. PubMed
  189. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  190. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  191. Xu X, et al. 2018. Cell. 175:1336. PubMed
  192. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  193. Bei T, et al. 2021. Molecules. 26:. PubMed
  194. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  195. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  196. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  197. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  198. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  199. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  200. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  201. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  202. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  203. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  204. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  205. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  206. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  207. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  208. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  209. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  210. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  211. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  212. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  213. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  214. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  215. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  216. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  217. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  218. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  219. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  220. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  221. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  222. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  223. Koide S, et al. 2022. iScience. 25:103603. PubMed
  224. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  225. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  226. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  227. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  228. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  229. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  230. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  231. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  232. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  233. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  234. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  235. Wang X, et al. 2022. Elife. 11:. PubMed
  236. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  237. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  238. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  239. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  240. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  241. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  242. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  243. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  244. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  245. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  246. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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