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APC anti-mouse CD45.2 Antibody APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg SEK278
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109814 100 µg SEK975
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  4. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  5. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  6. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  7. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  8. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  9. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  10. He D, et al. 2022. Immunity. 55:159. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Campisi L, et al. 2022. Nature. 606:945. PubMed
  13. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  14. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  15. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  16. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  17. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  18. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  19. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  20. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  21. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  22. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  23. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  24. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  25. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  26. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  27. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  28. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  29. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  30. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  33. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  37. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  38. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  39. Lu SX, et al. 2021. Cell. . PubMed
  40. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  41. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  42. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  43. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  44. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  45. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  46. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  47. Marangoni F, et al. 2021. Cell. . PubMed
  48. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  49. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  50. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  51. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  52. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  53. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  56. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  57. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  58. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  59. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  61. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  62. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  63. Bei T, et al. 2021. Molecules. 26:. PubMed
  64. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  65. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  66. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  67. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  68. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  69. Markey K, et al. 2012. Blood. 119:5918. PubMed
  70. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  71. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  72. Xu X, et al. 2018. Cell. 175:1336. PubMed
  73. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  74. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  75. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  76. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  77. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  78. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  79. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  80. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  81. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  82. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  83. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  84. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  85. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  86. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  87. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  88. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  89. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  90. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  91. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  92. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  93. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  94. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  95. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  96. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  97. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  102. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  103. Koide S, et al. 2022. iScience. 25:103603. PubMed
  104. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  105. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  106. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  107. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  108. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  109. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  110. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  111. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  112. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  113. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  114. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  115. Wang X, et al. 2022. Elife. 11:. PubMed
  116. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  117. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  118. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  119. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  120. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  121. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  122. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  123. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  124. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  125. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  126. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  127. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  128. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  129. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  130. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  131. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  132. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  133. He D, et al. 2022. Immunity. 55:159. PubMed
  134. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  135. Campisi L, et al. 2022. Nature. 606:945. PubMed
  136. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  137. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  138. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  139. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  140. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  141. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  142. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  143. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  144. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  145. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  146. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  147. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  148. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  149. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  150. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  151. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  152. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  153. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  154. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  155. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  156. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  157. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  158. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  159. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  160. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  161. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  162. Lu SX, et al. 2021. Cell. . PubMed
  163. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  164. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  165. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  166. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  167. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  168. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  169. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  170. Marangoni F, et al. 2021. Cell. . PubMed
  171. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  172. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  173. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  174. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  175. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  176. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  179. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  180. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  181. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  182. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  184. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  185. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  186. Bei T, et al. 2021. Molecules. 26:. PubMed
  187. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  188. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  189. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  190. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  191. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  192. Markey K, et al. 2012. Blood. 119:5918. PubMed
  193. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  194. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  195. Xu X, et al. 2018. Cell. 175:1336. PubMed
  196. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  197. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  198. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  199. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  200. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  201. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  202. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  203. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  204. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  205. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  206. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  207. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  208. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  209. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  210. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  211. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  212. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  213. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  214. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  215. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  216. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  217. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  218. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  219. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  220. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  221. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  222. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  223. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  224. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  225. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  226. Koide S, et al. 2022. iScience. 25:103603. PubMed
  227. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  228. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  229. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  230. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  231. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  232. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  233. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  234. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  235. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  236. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  237. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  238. Wang X, et al. 2022. Elife. 11:. PubMed
  239. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  240. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  241. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  242. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  243. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  244. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  245. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  246. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg SEK278
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109814 100 µg SEK975
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  4. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  5. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  6. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  7. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  8. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  9. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  10. He D, et al. 2022. Immunity. 55:159. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Campisi L, et al. 2022. Nature. 606:945. PubMed
  13. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  14. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  15. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  16. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  17. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  18. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  19. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  20. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  21. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  22. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  23. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  24. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  25. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  26. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  27. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  28. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  29. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  30. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  33. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  37. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  38. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  39. Lu SX, et al. 2021. Cell. . PubMed
  40. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  41. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  42. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  43. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  44. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  45. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  46. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  47. Marangoni F, et al. 2021. Cell. . PubMed
  48. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  49. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  50. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  51. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  52. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  53. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  56. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  57. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  58. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  59. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  61. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  62. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  63. Bei T, et al. 2021. Molecules. 26:. PubMed
  64. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  65. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  66. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  67. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  68. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  69. Markey K, et al. 2012. Blood. 119:5918. PubMed
  70. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  71. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  72. Xu X, et al. 2018. Cell. 175:1336. PubMed
  73. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  74. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  75. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  76. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  77. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  78. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  79. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  80. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  81. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  82. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  83. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  84. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  85. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  86. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  87. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  88. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  89. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  90. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  91. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  92. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  93. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  94. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  95. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  96. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  97. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  102. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  103. Koide S, et al. 2022. iScience. 25:103603. PubMed
  104. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  105. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  106. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  107. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  108. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  109. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  110. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  111. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  112. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  113. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  114. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  115. Wang X, et al. 2022. Elife. 11:. PubMed
  116. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  117. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  118. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  119. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  120. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  121. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  122. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  123. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  124. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  125. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  126. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  127. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  128. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  129. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  130. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  131. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  132. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  133. He D, et al. 2022. Immunity. 55:159. PubMed
  134. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  135. Campisi L, et al. 2022. Nature. 606:945. PubMed
  136. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  137. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  138. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  139. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  140. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  141. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  142. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  143. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  144. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  145. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  146. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  147. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  148. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  149. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  150. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  151. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  152. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  153. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  154. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  155. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  156. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  157. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  158. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  159. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  160. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  161. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  162. Lu SX, et al. 2021. Cell. . PubMed
  163. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  164. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  165. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  166. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  167. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  168. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  169. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  170. Marangoni F, et al. 2021. Cell. . PubMed
  171. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  172. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  173. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  174. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  175. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  176. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  179. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  180. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  181. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  182. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  184. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  185. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  186. Bei T, et al. 2021. Molecules. 26:. PubMed
  187. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  188. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  189. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  190. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  191. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  192. Markey K, et al. 2012. Blood. 119:5918. PubMed
  193. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  194. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  195. Xu X, et al. 2018. Cell. 175:1336. PubMed
  196. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  197. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  198. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  199. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  200. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  201. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  202. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  203. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  204. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  205. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  206. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  207. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  208. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  209. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  210. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  211. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  212. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  213. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  214. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  215. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  216. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  217. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  218. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  219. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  220. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  221. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  222. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  223. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  224. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  225. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  226. Koide S, et al. 2022. iScience. 25:103603. PubMed
  227. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  228. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  229. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  230. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  231. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  232. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  233. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  234. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  235. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  236. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  237. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  238. Wang X, et al. 2022. Elife. 11:. PubMed
  239. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  240. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  241. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  242. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  243. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  244. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  245. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  246. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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