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FITC anti-mouse CD45.2 Antibody FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
109805 50 µg SEK454
Check Availability


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109806 500 µg SEK975
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  4. He D, et al. 2022. Immunity. 55:159. PubMed
  5. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  6. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  7. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  8. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  9. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  10. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  11. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  12. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  13. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  14. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  15. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  16. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  17. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  18. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  19. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  20. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  21. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  22. Burman A, et al. 2007. Blood. 110:1064. PubMed
  23. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  24. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  25. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  26. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  27. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  28. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  29. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  30. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  31. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  32. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  33. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  34. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  35. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  36. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  37. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  38. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  39. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  40. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  41. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  42. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  43. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  44. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  45. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  46. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  47. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  48. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  49. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  50. Li J, et al. 2020. Elife. 9:00. PubMed
  51. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  52. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  53. Wan X, et al. 2018. Nature. 560:107. PubMed
  54. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  55. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  56. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  57. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  58. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  59. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  60. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  61. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  62. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  63. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  64. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  65. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  66. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  67. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  68. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  69. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  70. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  71. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  72. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  73. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  74. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  75. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  76. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  77. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  78. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  79. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  80. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  81. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  82. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  83. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  84. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  85. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  86. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  87. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  88. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  89. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  90. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  91. Koide S, et al. 2022. iScience. 25:103603. PubMed
  92. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  93. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  94. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  95. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  96. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  97. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  98. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  99. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  100. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  101. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  102. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  103. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  104. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  105. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  106. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  107. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  108. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  109. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  110. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  111. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  112. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  113. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  114. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  115. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  116. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  117. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  118. He D, et al. 2022. Immunity. 55:159. PubMed
  119. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  120. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  121. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  122. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  123. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  124. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  125. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  126. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  127. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  128. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  129. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  130. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  131. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  132. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  133. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  134. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  135. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  136. Burman A, et al. 2007. Blood. 110:1064. PubMed
  137. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  138. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  139. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  140. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  141. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  142. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  143. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  144. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  145. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  146. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  147. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  148. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  149. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  150. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  151. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  152. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  153. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  154. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  155. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  156. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  157. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  158. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  159. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  160. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  161. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  162. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  163. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  164. Li J, et al. 2020. Elife. 9:00. PubMed
  165. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  166. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  167. Wan X, et al. 2018. Nature. 560:107. PubMed
  168. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  169. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  170. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  171. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  172. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  173. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  174. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  175. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  176. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  177. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  178. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  179. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  180. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  181. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  182. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  183. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  184. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  185. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  186. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  187. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  188. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  189. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  190. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  191. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  192. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  193. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  194. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  195. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  196. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  197. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  198. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  199. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  200. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  201. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  202. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  203. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  204. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  205. Koide S, et al. 2022. iScience. 25:103603. PubMed
  206. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  207. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  208. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  209. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  210. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  211. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  212. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  213. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  214. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  215. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  216. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  217. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  218. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  219. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  220. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  221. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  222. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  223. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  224. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  225. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  226. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  227. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  228. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
109805 50 µg SEK454
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109806 500 µg SEK975
Check Availability


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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  2. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  3. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  4. He D, et al. 2022. Immunity. 55:159. PubMed
  5. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  6. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  7. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  8. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  9. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  10. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  11. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  12. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  13. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  14. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  15. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  16. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  17. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  18. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  19. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  20. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  21. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  22. Burman A, et al. 2007. Blood. 110:1064. PubMed
  23. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  24. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  25. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  26. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  27. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  28. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  29. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  30. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  31. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  32. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  33. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  34. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  35. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  36. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  37. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  38. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  39. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  40. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  41. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  42. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  43. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  44. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  45. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  46. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  47. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  48. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  49. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  50. Li J, et al. 2020. Elife. 9:00. PubMed
  51. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  52. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  53. Wan X, et al. 2018. Nature. 560:107. PubMed
  54. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  55. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  56. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  57. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  58. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  59. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  60. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  61. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  62. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  63. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  64. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  65. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  66. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  67. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  68. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  69. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  70. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  71. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  72. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  73. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  74. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  75. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  76. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  77. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  78. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  79. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  80. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  81. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  82. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  83. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  84. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  85. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  86. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  87. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  88. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  89. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  90. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  91. Koide S, et al. 2022. iScience. 25:103603. PubMed
  92. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  93. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  94. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  95. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  96. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  97. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  98. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  99. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  100. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  101. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  102. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  103. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  104. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  105. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  106. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  107. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  108. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  109. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  110. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  111. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  112. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  113. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  114. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  115. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  116. Behr FM, et al. 2021. Eur J Immunol. 51:151. PubMed
  117. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  118. He D, et al. 2022. Immunity. 55:159. PubMed
  119. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  120. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  121. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  122. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  123. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  124. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  125. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  126. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  127. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  128. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  129. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  130. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  131. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  132. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  133. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  134. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  135. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  136. Burman A, et al. 2007. Blood. 110:1064. PubMed
  137. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  138. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  139. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  140. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  141. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  142. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  143. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  144. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  145. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  146. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  147. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  148. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  149. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  150. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  151. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  152. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  153. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  154. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  155. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  156. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  157. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  158. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  159. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  160. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  161. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  162. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  163. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  164. Li J, et al. 2020. Elife. 9:00. PubMed
  165. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  166. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  167. Wan X, et al. 2018. Nature. 560:107. PubMed
  168. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  169. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  170. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  171. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  172. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  173. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  174. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  175. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  176. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  177. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  178. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  179. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  180. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  181. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  182. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  183. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  184. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  185. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  186. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  187. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  188. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  189. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  190. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  191. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  192. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  193. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  194. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  195. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  196. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  197. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  198. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  199. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  200. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  201. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  202. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  203. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  204. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  205. Koide S, et al. 2022. iScience. 25:103603. PubMed
  206. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  207. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  208. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  209. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  210. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  211. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  212. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  213. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  214. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  215. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  216. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  217. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  218. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  219. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  220. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  221. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  222. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  223. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  224. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  225. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  226. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  227. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  228. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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