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PE anti-mouse CD45.1 Antibody PE anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20
SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
  • A20
    SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
110708 200 µg SEK1798
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110707 50 µg SEK672
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  2. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  3. Albayrak E, et al. 2022. J Cell Biochem. 123:2009. PubMed
  4. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  5. Hägglöf T, et al. 2023. Cell. 186:147. PubMed
  6. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  7. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  8. Li X, et al. 2023. J Clin Invest. 133:. PubMed
  9. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  10. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  11. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  12. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  13. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  14. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  15. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  16. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  17. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  18. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  19. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  20. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  21. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  22. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  23. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  24. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  25. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  26. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  27. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  28. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  29. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  30. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  31. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  32. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  33. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  34. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  35. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  36. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  37. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  38. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  39. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  40. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  41. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  42. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  43. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  44. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  45. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  46. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  47. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  48. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  49. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  50. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  51. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  52. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  53. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  54. Bei T, et al. 2021. Molecules. 26:. PubMed
  55. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  56. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  57. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  58. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  59. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  60. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  61. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  62. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  63. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  64. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  65. Ma K, et al. 2022. iScience. 25:104347. PubMed
  66. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  67. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  68. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  69. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  70. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  71. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  72. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  73. Danzer H, et al. 2020. Elife. 9:00. PubMed
  74. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  75. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  76. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  77. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  78. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  79. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  80. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  81. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  82. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  83. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  84. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  85. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  86. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  87. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  88. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  89. Weiberg D, et al. 2018. PLoS One. 13:e0205247. PubMed
  90. Albayrak E, et al. 2022. J Cell Biochem. 123:2009. PubMed
  91. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  92. Hägglöf T, et al. 2023. Cell. 186:147. PubMed
  93. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  94. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  95. Li X, et al. 2023. J Clin Invest. 133:. PubMed
  96. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  97. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  98. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  99. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  100. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  101. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  102. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  103. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  104. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  105. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  106. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  107. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  108. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  109. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  110. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  111. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  112. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  113. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  114. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  115. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  116. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  117. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  118. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  119. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  120. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  121. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  122. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  123. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  124. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  125. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  126. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  127. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  128. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  129. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  130. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  131. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  132. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  133. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  134. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  135. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  136. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  137. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  138. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  139. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  140. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  141. Bei T, et al. 2021. Molecules. 26:. PubMed
  142. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  143. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  144. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  145. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  146. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  147. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  148. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  149. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  150. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  151. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  152. Ma K, et al. 2022. iScience. 25:104347. PubMed
  153. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  154. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  155. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  156. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  157. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  158. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  159. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  160. Danzer H, et al. 2020. Elife. 9:00. PubMed
  161. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  162. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  163. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  164. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  165. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  166. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  167. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  168. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  169. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  170. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  171. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  172. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  173. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  174. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_313497 (BioLegend Cat. No. 110708)
AB_313496 (BioLegend Cat. No. 110707)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC/Cyanine7 anti-mouse CD45.1 A20 FC
APC anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A20
SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
  • A20
    SJL (solid line) and C57BL/6 (broken line) splenocytes stained with A20 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
110708 200 µg SEK1798
Check Availability


Need larger quantities of this item?
Request Bulk Quote
110707 50 µg SEK672
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
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Product Citations
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  15. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  16. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
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  91. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  92. Hägglöf T, et al. 2023. Cell. 186:147. PubMed
  93. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  94. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
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  96. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  97. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  98. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  99. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  100. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  101. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  102. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  103. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  104. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  105. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  106. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  107. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  108. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  109. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  110. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  111. Bromley SK, et al. 2020. Cell Reports. 32(9):108085. PubMed
  112. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  113. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  114. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  115. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  116. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  117. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  118. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  119. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  120. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  121. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  122. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  123. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  124. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  125. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  126. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  127. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  128. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  129. Hu Z, et al. 2017. Sci Rep. 7:41558. PubMed
  130. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  131. Tang Y, et al. 2015. Exp Hematol. 43: 554-564. PubMed
  132. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  133. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  134. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  135. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  136. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  137. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  138. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  139. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  140. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  141. Bei T, et al. 2021. Molecules. 26:. PubMed
  142. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  143. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  144. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  145. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  146. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  147. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  148. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  149. Li C, et al. 2016. Nat Commun. 7:12632. PubMed
  150. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  151. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  152. Ma K, et al. 2022. iScience. 25:104347. PubMed
  153. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  154. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  155. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  156. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  157. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  158. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  159. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  160. Danzer H, et al. 2020. Elife. 9:00. PubMed
  161. Li J, et al. 2011. Dev Comp Immunol. 35:872. PubMed
  162. Pfau S, et al. 2016. Genes Dev. 30: 1395 - 1408. PubMed
  163. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  164. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  165. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  166. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  167. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  168. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  169. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  170. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  171. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  172. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  173. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  174. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_313497 (BioLegend Cat. No. 110708)
AB_313496 (BioLegend Cat. No. 110707)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45.1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.1 A20 FC,IP,Block,IHC-F
PE anti-mouse CD45.1 A20 FC
FITC anti-mouse CD45.1 A20 FC
Biotin anti-mouse CD45.1 A20 FC
Purified anti-mouse CD45.1 (Maxpar® Ready) A20 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.1 A20 FC
Alexa Fluor® 594 anti-mouse CD45.1 A20 IHC-F
APC/Fire™ 750 anti-mouse CD45.1 A20 FC
TotalSeq™-A0178 anti-mouse CD45.1 A20 PG
TotalSeq™-B0178 anti-mouse CD45.1 A20 PG
TotalSeq™-C0178 anti-mouse CD45.1 A20 PG
APC/Cyanine7 anti-mouse CD45.1 A20 FC
APC anti-mouse CD45.1 A20 FC
Alexa Fluor® 488 anti-mouse CD45.1 A20 FC
Alexa Fluor® 647 anti-mouse CD45.1 A20 FC
Pacific Blue™ anti-mouse CD45.1 A20 FC
Alexa Fluor® 700 anti-mouse CD45.1 A20 FC
Brilliant Violet 650™ anti-mouse CD45.1 A20 FC
PerCP anti-mouse CD45.1 A20 FC
PerCP/Cyanine5.5 anti-mouse CD45.1 A20 FC
PE/Cyanine7 anti-mouse CD45.1 A20 FC
Brilliant Violet 421™ anti-mouse CD45.1 A20 FC
Brilliant Violet 570™ anti-mouse CD45.1 A20 FC
Brilliant Violet 605™ anti-mouse CD45.1 A20 FC
Brilliant Violet 711™ anti-mouse CD45.1 A20 FC
Brilliant Violet 510™ anti-mouse CD45.1 A20 FC
Brilliant Violet 785™ anti-mouse CD45.1 A20 FC
Spark UV™ 387 anti-mouse CD45.1 A20 FC
Spark Blue™ 550 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Red™ 718 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark Blue™ 574 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 581 anti-mouse CD45.1 (Flexi-Fluor™) A20 FC
Spark YG™ 593 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
Spark NIR™ 685 anti-mouse CD45.1 (Flexi-Fluor™) Antibody A20 FC
StarBright UltraViolet 795 anti-mouse CD45.1 A20 FC
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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