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APC anti-mouse CD45.2 Antibody APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg NOK273
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109814 100 µg NOK957
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  3. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  4. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  5. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  6. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  7. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  8. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  9. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  10. He D, et al. 2022. Immunity. 55:159. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Campisi L, et al. 2022. Nature. 606:945. PubMed
  13. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  14. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  15. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  16. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  17. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  18. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  19. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  20. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  21. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  22. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  23. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  24. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  25. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  26. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  27. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  28. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  29. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  30. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  33. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  37. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  38. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  39. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  40. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  41. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  42. Lu SX, et al. 2021. Cell. . PubMed
  43. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  44. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  45. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  46. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  47. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  48. Marangoni F, et al. 2021. Cell. . PubMed
  49. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  50. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  51. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  52. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  53. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  56. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  57. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  58. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  59. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  61. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  62. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  63. Markey K, et al. 2012. Blood. 119:5918. PubMed
  64. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  65. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  66. Xu X, et al. 2018. Cell. 175:1336. PubMed
  67. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  68. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  69. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  70. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  71. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  72. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  73. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  74. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  75. Bei T, et al. 2021. Molecules. 26:. PubMed
  76. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  77. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  78. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  79. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  80. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  81. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  82. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  83. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  84. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  85. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  86. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  87. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  88. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  89. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  90. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  91. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  92. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  93. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  94. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  95. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  96. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  97. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  102. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  103. Koide S, et al. 2022. iScience. 25:103603. PubMed
  104. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  105. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  106. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  107. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  108. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  109. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  110. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  111. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  112. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  113. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  114. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  115. Wang X, et al. 2022. Elife. 11:. PubMed
  116. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  117. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  118. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  119. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  120. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  121. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  122. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  123. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  124. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  125. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  126. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  127. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  128. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  129. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  130. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  131. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  132. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  133. He D, et al. 2022. Immunity. 55:159. PubMed
  134. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  135. Campisi L, et al. 2022. Nature. 606:945. PubMed
  136. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  137. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  138. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  139. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  140. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  141. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  142. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  143. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  144. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  145. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  146. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  147. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  148. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  149. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  150. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  151. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  152. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  153. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  154. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  155. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  156. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  157. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  158. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  159. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  160. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  161. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  162. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  163. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  164. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  165. Lu SX, et al. 2021. Cell. . PubMed
  166. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  167. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  168. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  169. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  170. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  171. Marangoni F, et al. 2021. Cell. . PubMed
  172. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  173. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  174. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  175. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  176. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  179. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  180. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  181. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  182. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  184. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  185. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  186. Markey K, et al. 2012. Blood. 119:5918. PubMed
  187. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  188. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  189. Xu X, et al. 2018. Cell. 175:1336. PubMed
  190. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  191. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  192. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  193. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  194. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  195. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  196. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  197. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  198. Bei T, et al. 2021. Molecules. 26:. PubMed
  199. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  200. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  201. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  202. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  203. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  204. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  205. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  206. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  207. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  208. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  209. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  210. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  211. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  212. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  213. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  214. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  215. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  216. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  217. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  218. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  219. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  220. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  221. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  222. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  223. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  224. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  225. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  226. Koide S, et al. 2022. iScience. 25:103603. PubMed
  227. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  228. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  229. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  230. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  231. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  232. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  233. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  234. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  235. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  236. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  237. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  238. Wang X, et al. 2022. Elife. 11:. PubMed
  239. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  240. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  241. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  242. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  243. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  244. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  245. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  246. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
109813 25 µg NOK273
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109814 100 µg NOK957
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  3. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  4. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  5. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  6. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  7. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  8. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  9. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  10. He D, et al. 2022. Immunity. 55:159. PubMed
  11. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  12. Campisi L, et al. 2022. Nature. 606:945. PubMed
  13. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  14. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  15. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  16. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  17. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  18. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  19. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  20. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  21. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  22. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  23. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  24. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  25. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  26. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  27. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  28. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  29. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  30. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  31. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  32. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  33. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  37. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  38. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  39. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  40. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  41. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  42. Lu SX, et al. 2021. Cell. . PubMed
  43. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  44. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  45. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  46. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  47. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  48. Marangoni F, et al. 2021. Cell. . PubMed
  49. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  50. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  51. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  52. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  53. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  54. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  55. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  56. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  57. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  58. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  59. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  60. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  61. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  62. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  63. Markey K, et al. 2012. Blood. 119:5918. PubMed
  64. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  65. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  66. Xu X, et al. 2018. Cell. 175:1336. PubMed
  67. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  68. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  69. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  70. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  71. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  72. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  73. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  74. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  75. Bei T, et al. 2021. Molecules. 26:. PubMed
  76. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  77. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  78. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  79. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  80. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  81. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  82. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  83. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  84. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  85. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  86. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  87. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  88. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  89. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  90. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  91. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  92. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  93. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  94. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  95. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  96. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  97. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  99. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  100. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  101. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  102. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  103. Koide S, et al. 2022. iScience. 25:103603. PubMed
  104. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  105. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  106. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  107. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  108. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  109. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  110. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  111. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  112. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  113. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  114. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  115. Wang X, et al. 2022. Elife. 11:. PubMed
  116. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  117. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  118. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  119. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  120. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  121. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  122. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  123. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  124. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  125. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  126. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  127. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  128. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  129. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  130. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  131. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  132. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  133. He D, et al. 2022. Immunity. 55:159. PubMed
  134. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  135. Campisi L, et al. 2022. Nature. 606:945. PubMed
  136. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  137. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  138. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  139. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  140. Merchak AR, et al. 2023. PLoS Biol. 21:e3002000. PubMed
  141. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  142. Pan C, et al. 2022. Cell Mol Immunol. 19:516. PubMed
  143. Shirai T, et al. 2022. Cancer Sci. 113:3710. PubMed
  144. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  145. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  146. Ximerakis M, et al. 2023. Nat Aging. 3:327. PubMed
  147. Buckley MT, et al. 2023. Nat Aging. 3:121. PubMed
  148. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  149. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  150. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  151. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  152. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  153. Santamaria-Martínez A, et al. 2009. Exp Cell Res. 315:3004. PubMed
  154. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  155. Stanzione M, et al. 2022. Sci Adv. 8:eabn1229. PubMed
  156. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  157. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  158. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  159. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  160. Liu W, et al. 2021. Cell Mol Immunol. 18:2660. PubMed
  161. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  162. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  163. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  164. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  165. Lu SX, et al. 2021. Cell. . PubMed
  166. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  167. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  168. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  169. Han CY, et al. 2020. Cell Reports. 31(13):107818. PubMed
  170. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  171. Marangoni F, et al. 2021. Cell. . PubMed
  172. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  173. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  174. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  175. Osman A, et al. 2021. Nat Immunol. 22:1152. PubMed
  176. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  177. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  178. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  179. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  180. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  181. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  182. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
  183. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  184. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  185. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  186. Markey K, et al. 2012. Blood. 119:5918. PubMed
  187. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
  188. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  189. Xu X, et al. 2018. Cell. 175:1336. PubMed
  190. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  191. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  192. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  193. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  194. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  195. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  196. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  197. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  198. Bei T, et al. 2021. Molecules. 26:. PubMed
  199. Schaefer K, et al. 2014. PLoS One. 9:114824. PubMed
  200. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  201. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  202. Qin Q, et al. 2021. Cell Reports. 35(8):109161. PubMed
  203. Lim JE, et al. 2022. Front Oncol. 12:1045016. PubMed
  204. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  205. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  206. Chen X, et al. 2020. Am J Pathol. 190:2056. PubMed
  207. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  208. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  209. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  210. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  211. Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed
  212. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  213. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  214. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  215. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  216. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  217. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  218. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  219. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  220. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  221. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  222. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  223. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  224. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  225. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  226. Koide S, et al. 2022. iScience. 25:103603. PubMed
  227. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  228. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  229. Mandal RK, et al. 2020. BMC Biol. 83:18. PubMed
  230. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  231. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  232. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  233. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  234. Rivet-Noor CR, et al. 2022. Sci Rep. 12:8594. PubMed
  235. Wu B, et al. 2022. Nat Commun. 13:2155. PubMed
  236. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  237. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  238. Wang X, et al. 2022. Elife. 11:. PubMed
  239. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  240. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  241. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  242. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  243. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  244. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  245. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  246. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 581 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark YG™ 593 anti-mouse CD45.2 (Flexi-Fluor™) Antibody 104 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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